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rabbit igg isotype control  (Proteintech)


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    Proteintech rabbit igg isotype control
    Rabbit Igg Isotype Control, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isotype+controls/IgG+control+Antibody/pm42823744-100-0-6
    Average 96 stars, based on 1289 article reviews
    rabbit igg isotype control - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.

    Negative Control:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.


    Control:

    Article Title: Lipopolysaccharides derived from Porphyromonas gingivalis and Escherichia coli: Differential and interactive effects on novelty-induced hyperlocomotion, blood cytokine levels and TLR4-related processes.
    Article Snippet: Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1: Biolegend, San Diego, CA, USA) and Phycoerythrin (PE)-conjugated anti-mouse CD3 (17A2: Proteintech, Rosemont, IL, USA). .. Isotype controls used were FITC-labelled Rat IgG2b Isotype Control (LTF-2) (Proteintech, Rosemont, IL, USA) and PE-labelled Rat IgG2b Isotype Control (Becton Dickinson Pharmingen, San Jose, CA, USA). ..


    Article Title: Lipopolysaccharides derived from Porphyromonas gingivalis and Escherichia coli : Differential and interactive effects on novelty-induced hyperlocomotion, blood cytokine levels and TLR4-related processes
    Article Snippet: Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1: Biolegend, San Diego, CA, USA) and Phycoerythrin (PE)-conjugated anti-mouse CD3 (17A2: Proteintech, Rosemont, IL, USA). .. Isotype controls used were FITC-labelled Rat IgG2b Isotype Control (LTF-2) (Proteintech, Rosemont, IL, USA) and PE-labelled Rat IgG2b Isotype Control (Becton Dickinson Pharmingen, San Jose, CA, USA). ..

    Article Title: PD-L2 regulates natural antibody and IL-10 secretion by B-1 cells
    Article Snippet: After washing with perm buffer cells were resuspended in FACS buffer (1% BSA PBS) and acquired using a BD Fortessa flow cytometer. .. Intracellular antibodies used, indicating antigen and fluorophore (clone): Blimp-1 APC (5E7) and IRF4 PE (IRF4.3E4) (both BioLegend), CstF-64 CL488 (rabbit polyclonal) (Proteintech); Isotype controls: Rat IgG2a APC isotype control (G013C12), rat IgG1 PE isotype control (G0114F7) (both BioLegend), rabbit IgG1 AF488 (EPR25A; BD Biosciences). .. To measure antibody levels in serum, blood was collected by heart puncture and serum obtained by spin-separation of blood and serum using gel serum collection tubes (SAI Infusion Technologies).



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    Image Search Results


    The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biomedical Journal

    Article Title: Anti-PD-L1 improves barrier function and reduces CD103 + DC cell accumulation by regulating gut and lung microbiota and its metabolites to alleviate asthma in juvenile mice

    doi: 10.1016/j.bj.2025.100944

    Figure Lengend Snippet: The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Based on previous [ , , ] studies with appropriate modifications and our previous pre-experimental protocol, the anti-mouse PD-L1 monoclonal antibody (10 μg/g/mouse, BioXcell, 804722F1B, America) and rat IgG2b (Clone: LTF-2) isotype control antibody (10 μg/g/mouse, BioXcell, 767921D1, America) were administered by i.p. injection on days 0, 7, and 14 of sensitization and 21, 28, and 35 of challenge.

    Techniques: Expressing